Must you test on-site with whole blood directly (no centrifuge)?
Yes → Eliminate HF04-3 (serum-only). Choose among HF04-1 / HF04-2 / HF04-4.
No → Have a centrifuge, can process serum? Consider HF04-3 for earliest detection (Day 25) and lowest cost.
How to choose bovine pregnancy rapid test?
2026-07-27 01:19 Kevin Xiong
Bovine Early Pregnancy Rapid Test — Complete Product Selection Guide
Four Herun Biopharm configurations (HF04-1 / HF04-2 / HF04-3 / HF04-4) compared side-by-side. Use this guide to choose the right kit based on sample type, interpretation method, packaging, and field scenario.
What this guide covers: All four Herun Biopharm configurations of the Bovine Early Pregnancy Rapid Test. Each kit detects Pregnancy-Associated Glycoproteins (PAG) via colloidal-gold immunochromatography, but they differ in (1) sample type — whole blood vs serum; (2) interpretation — three-line CBT vs two-line CT; and (3) packaging — bulk box vs single-dose pouch. The right choice depends on where the test is performed (lab or on-farm), the skill level of the operator, and your need for early detection vs cost-effectiveness.
1. Product Images
2. At-a-Glance Product Comparison
3. Detailed Product Profiles
4. Detailed Selection Criteria
4.1 Sample Type → "Where can you test?"
Whole-Blood Versions
HF04-1 · HF04-2 · HF04-4
Accept whole blood, serum, plasma.
No centrifuge required — true "on-site / on-farm" testing.
Specimens: fresh or stored at 2 – 8 °C, use within 3 days.
Earliest reliable detection: Day 28 post-breeding.
Best for streamlining workflow and reducing equipment dependence.
Serum / Plasma Version
HF04-3
Accept serum / plasma only.
Requires centrifugation (extra 10 – 15 min prep).
Higher analytical sensitivity → Day 25 earliest detection.
Ideal for laboratory or fixed testing station with trained staff.
Lowest unit cost among the four configurations.
Sample Volume & Procedure
Whole blood: 2 drops (~60 µL) + 3 drops buffer; wait 5 sec before buffer.
Serum / Plasma (whole-blood kits): 1 drop (~30 µL) + 3 drops buffer.
Serum / Plasma (HF04-3): 4 drops (~120 µL) directly; no buffer.
Read results at 10 – 20 min; results after 20 min are invalid.
4.2 Interpretation Method → "How do you read results?"
This is the most critical technical factor affecting real-world accuracy.
Two-line (CT)
HF04-3 · HF04-4
Format: T line (test) + C line (control). Simple "any T = positive" read.
Pregnant: C line + T line (no matter how faint).
Open: C line only.
Invalid: No C line.
⚠️ The challenge: weak T lines near the cutoff can be read differently by different operators.
Three-line (CBT)
HF04-1 · HF04-2
Format: C (control) + B (built-in color reference) + T (test). T line compared to B line.
Not pregnant: T lighter than B, or T not visible.
Indeterminate: T similar to B → retest after 7 days.
Pregnant: T significantly darker than B, or T clearly visible while B is faint.
✅ Minimizes subjective error from weak positives — ideal for mixed-skill teams.
Which Should You Choose?
Reduce human error with mixed-skill teams → CBT (HF04-1 / HF04-2).
Simplest "yes/no" read with experienced operators → CT (HF04-4).
Earliest detection with lab conditions → CT HF04-3 (serum).
Objective interpretation to minimize disputes → CBT versions.
4.3 Packaging Format → "What's included in the box?"
Boxed Versions
HF04-1 · HF04-3 · HF04-4
Available in 1 / 10 / 50 / 80 tests per box.
Each cassette in a foil pouch with desiccant.
Kit includes cassettes, droppers, assay buffer (whole-blood kits), and a paper IFU.
Designed for fixed-site bulk testing.
Pouch Version
HF04-2
Single test per foil pouch.
IFU printed directly on the pouch — no separate paper insert.
Includes sampling accessories (EDTA tube + syringe) so the user does not need to bring any tools.
Designed for mobile / out-call / field scenarios.
Common Specifications
Assay time: 10 – 20 minutes (read window).
Storage: 2 – 30 °C (room temperature).
Shelf life: 24 months from manufacture.
Optimal testing temperature: 15 – 45 °C (59 – 113 °F).
Principle: double-antibody sandwich + colloidal gold chromatography (PAG detection).
Note on HF04-2 packaging: HF04-2 does not ship with a separate paper IFU. The complete instructions — intended use, sample procedure, interpretation, precautions — are printed on the back of each individual foil pouch. This makes the pouch format ideal for veterinary field bags where loose paper inserts can be lost.
5. Quick Decision Flowchart
1
2
Among whole-blood versions, do you want two-line (CT) or three-line (CBT)?
Less experienced team, want objective reading, want to reduce disputes → Choose CBTHF04-1 (box) or HF04-2 (pouch).
Experienced team, confident in reading weak lines, cost-sensitive → Choose CTHF04-4 (most economical whole-blood option).
3
Do you need a complete self-contained sampling kit for mobile / out-call use?
Yes → Frequent out-calls or no on-site supplies → choose pouch HF04-2 (IFU printed on pouch + EDTA tube + syringe included).
No → On-farm with basic supplies already available → choose boxed HF04-1 (CBT) or HF04-4 (CT).
6. Final Recommendations
★
Best All-Rounder → HF04-1 (Box, Whole Blood, Three-line CBT)
Balances convenience (whole blood, no centrifuge), accuracy (built-in B-line for objective reading), and value. The most reliable choice for most herds and mixed-skill teams.
✓
Best Value / Cost-Effective → HF04-4 (Box, Whole Blood, Two-line CT)
Most economical whole-blood option. Choose this if your team is experienced and can confidently interpret weak positive T lines without the B-line reference.
⏱
Earliest Detection / Lab Use → HF04-3 (Box, Serum, Two-line CT)
Enables testing from Day 25 post-breeding — 3 days earlier than whole-blood versions. Most economical configuration. Requires centrifuge and trained lab personnel.
🚐
Mobile / Field Veterinarian → HF04-2 (Pouch, Whole Blood, Three-line CBT)
Self-contained kit with IFU printed on the pouch plus complete sampling accessories (EDTA tube + syringe). Designed for out-call and field-bag use.
7. Technical Notes & Precautions
Common to all HF04 kits:
Specimens (whole blood / serum / plasma) can be stored at 2 – 8 °C and used within 3 days.
Allow all materials and the test device to reach 15 – 25 °C before running the assay.
Read results at 10 – 20 minutes; results after 20 minutes are invalid.
Once the foil pouch is opened, the test cassette should be used within 1 hour.
Storage: 2 – 30 °C. Shelf life 24 months. Do not freeze. Keep away from direct sunlight.
Important safety & accuracy notes:
This product is intended solely for early pregnancy detection in bovine and is not for diagnosing animal diseases.
Miscarriage or stillbirth can cause false-positive results. Risk factors include maternal malnutrition, mycotoxins in feed, illness, and trauma.
If the result is suspicious or indeterminate, retest after 7 – 10 days.
If the C line is absent, the test is invalid — repeat with a new cassette.
This product is for single use only — do not reuse cassettes or droppers.
Reading CBT results (HF04-1 / HF04-2) — quick reference:
Not pregnant: T-line color lighter than B-line, or T-line not visible. · Indeterminate: T-line color similar to B-line → retest after 7 days. · Pregnant: T-line significantly darker than B-line, or T-line clearly visible while B-line is faint. · Invalid: C line absent.
Not pregnant: T-line color lighter than B-line, or T-line not visible. · Indeterminate: T-line color similar to B-line → retest after 7 days. · Pregnant: T-line significantly darker than B-line, or T-line clearly visible while B-line is faint. · Invalid: C line absent.
Reading CT results (HF04-3 / HF04-4) — quick reference:
Pregnant: C band + T band present (no matter how faint the T band). · Not pregnant: Only clear C band appears; no T band. · Invalid: No color band appears in the C zone.
Pregnant: C band + T band present (no matter how faint the T band). · Not pregnant: Only clear C band appears; no T band. · Invalid: No color band appears in the C zone.